66059-1-IG

antibody from Invitrogen Antibodies

Targeting: RBM15 OTT, OTT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66059-1-IG

Provider

Invitrogen Antibodies

Product name

RBM15 Monoclonal Antibody (4A1A4)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: SSSRDRYNS DNDRSSRLLL ERPSPIRDRR GSLEKSQGDK RDRKNSASAE RDRKHRTTAP TEGKSPLKKE DRSDGSAPST STASSKLKSP SQKQDGGTAP VASASPKLCL AWQGMLLLKN SNFPSNMHLL QGDLQVASSL LVEGSTGGKV AQLKITQRLR LDQPKLDEVT RRIKVAGPNG YAILLAVPGS SDSRSSSSSA ASDTATSTQR PLRNLVSYLK QKQAAGVISL PVGGNKDKEN TGVLHAFPPC EFSQQFLDSP AKALAKSEED YLVMIIVRGF GFQIGVRYEN KKRENLALTL L (677-977 aa encoded by BC006397)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

4A1A4

Vial size

150 µL

Concentration

0.99 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 66059-1-IG (RBM15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of RBM15 antibody (66059-1-IG, 1:4000) with si-control and si-RBM15 transfected HEK293 cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 66059-1-Ig (RBM15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM15 antibody (Product # 66059-1-IG, Clone: 4A1A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( ) fixed Hela cells using 66059-1-IG (RBM15 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66059-1-Ig (RBM15 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66059-1-IG ( RBM15 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RBM15 (IP:66059-1-IG, 3ug; Detection:66059-1-IG 1:500) with HEK-293 cells lysate 1800ug.