Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00005358-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005358-M01, RRID:AB_10961952
- Product name
- PLS3 monoclonal antibody (M01), clone 5B9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PLS3.
- Antigen sequence
MDEMATTQISKDELDELKEAFAKVDLNSNGFICDY
ELHELFKEANMPLPGYKVREIIQKLMLDGDRNKDG
KISFDEFVYIFQEVKSSDIAKTFRKAINRKEGICA
LGGTSELSSEGTQHSYSEEEKYAFVNWINKALEND
PDCRHVIPMNPNTDDLFKAVGDGIVLCKMINLSVP
DTIDERAINKKKLTPFIIQENLNLALNSASAIGCH
VVNIGAEDLRAGKPHLVLGLLWQIIKIGLFADIEL
SRNEALAALLRDGETLEELMKLSPEELLLRWANFH
LENSGWQKINNFSADIKDSKAYFHLLNQIAPKGQK
EGEPRIDINMSGFNETDDLKRAESMLQQADKLGCR
QFVTPADVVSGNPKLNLAFVANLFNKYPALTKPEN
QDIDWTLLEGETREERTFRNWMNSLGVNPHVNHLY
ADLQDALVILQLYERIKVPVDWSKVNKPPYPKLGA
NMKKLENCNYAVELGKHPAKFSLVGIGGQDLNDGN
QTLTLALVWQLMRRYTLNVLEDLGDGQKANDDIIV
NWVNRTLSEAGKSTSIQSFKDKTISSSLAVVDLID
AIQPGCINYDLVKSGNLTEDDKHNNAKYAVSMARR
IGARVYALPEDLVEVKPKMVMTVFACLMGRGMKRV- Isotype
- IgG
- Antibody clone number
- 5B9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PLS3 monoclonal antibody (M01), clone 5B9. Western Blot analysis of PLS3 expression in MCF-7.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PLS3 monoclonal antibody (M01), clone 5B9. Western Blot analysis of PLS3 expression in MDA-MB-231.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PLS3 expression in transfected 293T cell line by PLS3 monoclonal antibody (M01), clone 5B9.Lane 1: PLS3 transfected lysate (Predicted MW: 70.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PLS3 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PLS3 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
