12917-1-AP

antibody from Invitrogen Antibodies

Targeting: PLS3 T-plastin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12917-1-AP

Provider

Invitrogen Antibodies

Product name

PLS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: LENSGWQKI NNFSADIKDS KAYFHLLNQI APKGQKEGEP RIDINMSGFN ETDDLKRAES MLQQADKLGC RQFVTPADVV SGNPKLNLAF VANLFNKYPA LTKPENQDID WTLLEGETRE ERTFRNWMNS LGVNPHVNHL YADLQDALVI LQLYERIKVP VDWSKVNKPP YPKLGANMKK LENCNYAVEL GKHPAKFSLV GIGGQDLNDG NQTLTLALVW QLMRRYTLNV LEDLGDGQKA NDDIIVNWVN RTLSEAGKST SIQSFKDKTI SSSLAVVDLI DAIQPGCINY DLVKSGNLTE DDKHNNAKYA VSMARRIGAR VYALPEDLVE VKPKMVMTVF ACLMGRGMKR V (281-630 aa encoded by BC039049)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.34 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 12917-1-AP ( PLS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 12917-1-AP (PLS3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of A431 cells using 12917-1-AP (PLS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PLS3 (IP:12917-1-AP, 4ug; Detection:12917-1-AP 1:300) with A431 cells lysate 2400ug.