PA5-27883

antibody from Invitrogen Antibodies

Targeting: PLS3 T-plastin

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27883

Provider

Invitrogen Antibodies

Product name

PLS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, JurKat, Raji, NIH-3T3, JC, BCL-1.

Concentration

0.23 mg/mL

References

Submitted references

Cul3 regulates cytoskeleton protein homeostasis and cell migration during a critical window of brain development.

Morandell J, Schwarz LA, Basilico B, Tasciyan S, Dimchev G, Nicolas A, Sommer C, Kreuzinger C, Dotter CP, Knaus LS, Dobler Z, Cacci E, Schur FKM, Danzl JG, Novarino G
Nature communications 2021 May 24;12(1):3058

---

T-Plastin reinforces membrane protrusions to bridge matrix gaps during cell migration.

Garbett D, Bisaria A, Yang C, McCarthy DG, Hayer A, Moerner WE, Svitkina TM, Meyer T
Nature communications 2020 Sep 23;11(1):4818

---

Evaluation of the role of an antioxidant gene in NSC-34 motor neuron-like cells as a model of a motor neuron disease.

Alrafiah AR
Folia morphologica 2019;78(1):1-9

---

The Actin Binding Protein Plastin-3 Is Involved in the Pathogenesis of Acute Myeloid Leukemia.

Velthaus A, Cornils K, Hennigs JK, Grüb S, Stamm H, Wicklein D, Bokemeyer C, Heuser M, Windhorst S, Fiedler W, Wellbrock J
Cancers 2019 Oct 26;11(11)

---

Circulating tumour cell-derived plastin3 is a novel marker for predicting long-term prognosis in patients with breast cancer.

Ueo H, Sugimachi K, Gorges TM, Bartkowiak K, Yokobori T, Müller V, Shinden Y, Ueda M, Ueo H, Mori M, Kuwano H, Maehara Y, Ohno S, Pantel K, Mimori K
British journal of cancer 2015 Apr 28;112(9):1519-26

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of T-Plastin using 30 µg of A) HeLa S3 and B) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a T-Plastin polyclonal antibody (Product # PA5-27883) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PLS3 Polyclonal Antibody (Product # PA5-27883). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. Lane C: BCL-1. 7.5% SDS PAGE. PLS3 Polyclonal Antibody (Product # PA5-27883) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PLS3 Polyclonal Antibody detects T-Plastin protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PLS3 Polyclonal Antibody (Product # PA5-27883) diluted at a dilution of 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PLS3 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: PLS3 Polyclonal Antibody (Product # PA5-27883) diluted at 1:100. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PLS3 Polyclonal Antibody detects T-Plastin protein at cytoplasm on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. PLS3 Polyclonal Antibody (Product # PA5-27883) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2 Comparison of PLS3 expression in breast cancer cell lines with PLS3 expression in peripheral blood mononuclear cells of healthy control individuals by immunocytochemical double staining. Cells of the assigned cell lines were spiked in the blood samples. Identification of the tumour cells was supported by the cytokeratin-specific antibody AE1/AE3. Nuclei were stained with DAPI, and the composite image is an overlay of the DAPI, cytokeratin, and PLS3 images. Two different PLS3 antibodies were applied for the analysis. ( A ) For MDA-MB-468 (MDA-468), an enlarged view of stained cells is shown. ( B ) BC-M1 analysis for PLS3 and blood samples without cell spiking. PLS3 signals in PBMC were labelled with arrows.