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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-55019 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MYO10 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QRMKEQQELS LTEASLQKLQ ERRDQELRRL EEEACRAAQE FLESLNFDEI DECVRNIERS LSVGSEFSSE LAESACEEKP NFNFSQPYPE EEVDEGFEAD DDAFKDSPNP SEHGHSDQRT SGIRTSDDSS EEDPYMNDTV VPTSPSA
- Concentration
- 0.2 mg/mL
Submitted references Phenotypic and Functional Alterations in Tunneling Nanotubes Formed by Glaucomatous Trabecular Meshwork Cells.
In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.
Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.
Sun YY, Bradley JM, Keller KE
Investigative ophthalmology & visual science 2019 Nov 1;60(14):4583-4595
Investigative ophthalmology & visual science 2019 Nov 1;60(14):4583-4595
In situ molecular characterization of endoneurial microvessels that form the blood-nerve barrier in normal human adult peripheral nerves.
Ouyang X, Dong C, Ubogu EE
Journal of the peripheral nervous system : JPNS 2019 Jun;24(2):195-206
Journal of the peripheral nervous system : JPNS 2019 Jun;24(2):195-206
Myosin-X Silencing in the Trabecular Meshwork Suggests a Role for Tunneling Nanotubes in Outflow Regulation.
Sun YY, Yang YF, Keller KE
Investigative ophthalmology & visual science 2019 Feb 1;60(2):843-851
Investigative ophthalmology & visual science 2019 Feb 1;60(2):843-851
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MYO10 was achieved by transfecting HeLa with MYO10 specific siRNAs (Silencer® select Product # s9224, Product # s9223). Western blot analysis (Fig. a) was performed using whole cell extracts from the MYO10 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with MYO10 Polyclonal Antibody (Product # PA5-55019, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MYO10.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MYO10 Polyclonal Antibody (Product # PA5-55019) and bands around 240, 140, 110 kDa corresponding to MYO10 were observed in the cell lines tested. Whole cell extracts (40 µg lysate) of A549 (Lane 1) and HeLa (Lane 2) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # LC2001) by XCell SureLock™ Mini-Cell and XCell II™ Blot Module (Product # EI0002). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MYO10 in human cell line U-2 OS shows positivity in nucleoli, plasma membrane & cytoplasm. Samples were probed using a MYO10 Polyclonal Antibody (Product # PA5-55019).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of MYO10 in human cell line U-2 OS using a MYO10 Polyclonal Antibody (Product # PA5-55019) shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYO10 in human kidney using MYO10 Polyclonal Antibody (Product # PA5-55019) shows strong membranous positivity in cells in glomeruli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYO10 in human lymphoid tissues using MYO10 Polyclonal Antibody (Product # PA5-55019) shows weak cytoplasmic positivity in germinal center cells.
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYO10 in human placenta using MYO10 Polyclonal Antibody (Product # PA5-55019) shows moderate cytoplasmic positivity in trophoblastic cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of MYO10 in human cerebellum using MYO10 Polyclonal Antibody (Product # PA5-55019) shows moderate cytoplasmic positivity in Purkinje cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Peripheral nerve cell-specific structural proteins. Representative indirect fluorescent digital photomicrographs of cryostat axial sections of normal adult sural nerves show UEA-1-positive endothelial cells (green; A, E, I, M, Q) with expression of PCDH1, CD44, ACTG1, CALD1, and MYO10 (red; B, F, J, N, R, respectively) on endoneurial microvessels, fenestrated epineurial macrovessels, perineurium and Schwann cells (PCDH1) and axons (CD44, ACTG1, CALD1, and MYO10), shown in the merged images at lower and higher magnifications (yellow/orange for endothelial cells). Expression of these proteins by these cell types implies roles in maintaining the structural integrity of peripheral nerve-specific cells in normal adult nerves. White arrows demonstrate positively staining endoneurial microvessels. Blue (4, 6-diamidino-2-phenylindole) staining indicates nuclei. Scale bar 500 mum for A-C, E-G, I-K, M-O, and Q-S and 125 mum for D, H, L, P, and T
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Myo10 protein distribution in NTM and GTM cells. Myo10 (green) and SiR-actin (red) in two NTM cell strains (A-G) and two GTM cell strains (I-O). Myo10 colocalized with cortactin in NTM cells (H) and GTM cells (P). Scale bars: 20 mum. (Q) Western immunoblotting showing Myo10 protein levels in GTM (n = 2) and NTM cells (n = 4). (R) Densitometry of Western immunoblots (n = 19 technical replicates) of GTM (n = 6 biologic replicates) and NTM (n = 9 biologic replicates). (S) Pearson's colocalization values of Myo10 and cortactin in GTM (n = 35) and NTM (n = 19) cells. (T) The diameter of actin stress fibers was calculated in GTM (n = 195) and NTM (n = 200) cells. *P = 0.0001.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Generation of shRNA Myo10-silencing lentivirus (shMyo10) silencing lentivirus. Efficacy of Myo10 knockdown in human TM cells by infection with shMyo10-silencing lentivirus was tested by (A) quantitative RT-PCR using Myo10-specific primer sets. *P = 0.0001; n = 4 from 4 biological replicates. (B) Western immunoblotting of Myo10 with tubulin as a loading control and (C) densitometry of Myo10 knockdown. **P = 0.001; n = 7 from 5 biological replicates. Immunofluorescence of Myo10 in (D) shCtrl and (E) shMyo10-silenced human TM cells. Insets show DAPI staining. Scale bar: 20 mum.
