16448-1-AP

antibody from Invitrogen Antibodies

Targeting: DEK D6S231E

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16448-1-AP

Provider

Invitrogen Antibodies

Product name

DEK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MSASAPAAE GEGTPTQPAS EKEPEMPGPR EESEEEEDED DEEEEEEEKE KSLIVEGKRE KKKVERLTMQ VSSLQREPFT IAQGKGQKLC EIERIHFFLS KKKTDELRNL HKLLYNRPGT VSSLKKNVGQ FSGFPFEKGS VQYKKKEEML KKFRNAMLKS ICEVLDLERS GVNSELVKRI LNFLMHPKPS GKPLPKSKKT CSKGSKKERN SSGMARKAKR TKCPEILSDE SSSDEDEKKN KEESSDDEDK ESEEEPPKKT AKREKPKQKA TSKSKKSVKS ANVKKADSST TKKNQNSSKK ESESEDSSDD EPLIKKLKKP PTDEELKETI KKLLASANLE EVTMKQICKK VYENYPTYDL TERKDFIKTT VKELIS (1-375 aa encoded by B C035259)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.29 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Rat brain tissue were subjected to SDS PAGE followed by western blot with 16448-1-AP (DEK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DEK antibody (Product # 16448-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using DEK antibody 16448-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 16448-1-AP (DEK antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human brain using 16448-1-AP (DEK antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-DEK (IP:16448-1-AP, 4ug; Detection:16448-1-AP 1:500) with HeLa cells lysate 1200ug.