Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-117908 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DEK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot of DEK in Lane A: HeLa Whole Cell Lysate, Lane B: K562 Whole Cell Lysate. Samples (30 µg per lane) were incubated with polyclonal antibody (Product # PA5-117908) with a dilution of 1:500 , followed by Goat Anti-Rabbit IgG H&L (Dylight 800) using a dilution of 1:10,000. Assay was performed under reducing conditions. Predicted band size: 43 kDa, Observed band size: 50 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DEK Polyclonal Antibody (Product # PA5-117908) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti- Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 43 kDa. Observed band size: 50 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of DEK in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with DEK Polyclonal Antibody (Product # PA5-117908, 1:5,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of DEK in Lane A: 0.5 mg K562 Whole Cell Lysate. Samples were treated with 15 μl of 50 % Protein G agarose, incubated with polyclonal antibody (Product # PA5-117908) with a dilution of 1:500 , followed by Clean-Blot HRP Detection reagent using a dilution of 1:500. Assay was performed under reducing conditions. Predicted band size: 42 kDa , Observed band size: 42 kDa .
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DEK Immunoprecipitation using: Lane A: 0.5 mg K562 Whole Cell Lysate 1 µL with DEK Polyclonal Antibody (Product # PA5-117908) and 15 µL of 50 % Protein G agarose. Primary antibody: DEK Polyclonal Antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 42 kDa. Observed band size: 42 kDa.