Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- 51002-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IST1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MLGSGFKAE RLRVNLRLVI NRLKLLEKKK TELAQKARKE IADYLAAGKD ERARIRVEHI IREDYLVEAM EILELYCDLL LARFGLIQSM KELDSGLAES VSTLIWAAPR LQSEVAELKI VADQLCAKYS KEYGKLCRTN QIGTVNDRLM HKLSVEAPPK ILVERYLIEI AKNYNVPYEP DSVVMAEAPP GVETDLIDVG FTDDVKKGGP GRGGSGGFTA PVGGPDGTVP MPMPMPMPMP SANTPFSYPL PKGPSDFNGL PMGTYQAFPN IHPPQIPATP PSYESMTLML IRISLLHRLL VLDPSQKPLQ SFLPDLQITM TTLSYQSCHL CQTHYQLHLL VPAPQHLKTL TLMIFPGGLK S (1-364 aa encoded by BC000430)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 51002-1-AP (IST1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 51002-1-AP (IST1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 51002-1-AP (IST1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using KIAA0174 antibody 51002-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 51002-1-AP (IST1 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 51002-1-AP (IST1 antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 51002-1-AP) (hIST1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).