PA5-119936

antibody from Invitrogen Antibodies

Targeting: ATP1B4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119936

Provider

Invitrogen Antibodies

Product name

ATP1B4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Human skeletal muscle tissue lysate, mouse skeletal muscle tissue lysate, mouse tongue tissue lysate, 293 cell lysate, mouse tongue tissue.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP1B4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. ATP1B4 Polyclonal Antibody (Product # PA5-119936) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skeletal muscle tissue lysate. Lane 2: Mouse skeletal muscle tissue lysate. Lane 3: Mouse tongue tissue lysate. Lane 4: 293 cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ATP1B4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. ATP1B4 Polyclonal Antibody (Product # PA5-119936) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skeletal muscle tissue lysate. Lane 2: Mouse skeletal muscle tissue lysate. Lane 3: Mouse tongue tissue lysate. Lane 4: 293 cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded mouse tongue tissue using ATP1B4 Polyclonal Antibody (Product # PA5-119936). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ATP1B4 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.