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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 66656-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT2 Monoclonal Antibody (3H7B10)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: MNAPLGGIW LWLPLLLTWL TPEVNSSWWY MRATGGSSRV MCDNVPGLVS SQRQLCHRHP DVMRAISQGV AEWTAECQHQ FRQHRWNCNT LDRDHSLFGR VLLRSSRESA FVYAISSAGV VFAITRACSQ GEVKSCSCDP KKMGSAKDSK GIFDWGGCSD NIDYGIKFAR AFVDAKERKG KDARALMNLH NNRAGRKAVK RFLKQECKCH GVSGSCTLRT CWLAMADFRK TGDYLWRKYN GAIQVVMNQD GTGFTVANER FKKPTKNDLV YFENSPDYCI RDREAGSLGT AGRVCNLTSR GMDSCEVMCC GRGYDTSHVT RMTKCGCKFH WCCAVRCQDC LEALDVHTCK APKNADWTTA T (1-360 aa encoded by BC029854)
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3H7B10
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-IG (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66656-1-IG (WNT2 antibody) at dilution of 1:44000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 66656-1-IG (WNT2 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
