MA5-42889

antibody from Invitrogen Antibodies

Targeting: IKZF3 Aiolos, ZNFN1A3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42889

Provider

Invitrogen Antibodies

Product name

IKZF3 Recombinant Rabbit Monoclonal Antibody (4U7F9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: Raji, Mouse spleen, Mouse thymus, Rat lung.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

4U7F9

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IKZF3 in Raji cells using a IKZF3 Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IKZF3 in Raji cells using a IKZF3 Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of IKZF3 in Raji cells using a IKZF3 Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 3s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of IKZF3 in mouse spleen using a IKZF3 Recombinant Monoclonal antibody (Product # MA5-42889). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of IKZF3 in mouse spleen using a IKZF3 Recombinant Monoclonal antibody (Product # MA5-42889). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IKZF3 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IKZF3 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IKZF3 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IKZF3 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of IKZF3 in human colon carcinoma using a Recombinant Monoclonal antibody (Product # MA5-42889) at a dilution of 1:100. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol..