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LearnA304-167A
antibody from Invitrogen Antibodies
Targeting: PPIP5K2
CFAP160, DFNB100, HISPPD1, KIAA0433, VIP2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Other assay [1]
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- Product number
- A304-167A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PPIP5K2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPIP5K2 by western blot. Samples: Whole cell lysate (10 µg) from K-562, GaMG, A-549, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (Product # A304-167A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse PPIP5K2 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PPIP5K2 antibody (Product # A304-167A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPIP5K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PPIP5K2 antibody A304-167A (lot A304-167A-1) used for IP at 6 µg/mg lysate. PPIP5K2 was also immunoprecipitated by rabbit anti-PPIP5K2 antibody A304-166A. For blotting immunoprecipitated PPIP5K2, A304-167A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse PPIP5K2 by western blot. Samples: Whole cell lysate (50 µg) from K562, HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-PPIP5K2 antibody A304-167A (lot A304-167A-1) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human PPIP5K2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPIP5K2 antibody (Product # A304-167A; Lot 2) used for IP at 6 µg per reaction. PPIP5K2 was also immunoprecipitated by a previous lot of this antibody (Product # A304-167A; Lot 1) and another rabbit anti-PPIP5K2 antibody (Product # A304-166A). For blotting immunoprecipitated PPIP5K2, Product # A304-167A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 75 seconds.
