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antibody from Invitrogen Antibodies
Targeting: CCT6A
CCT6, Cctz, HTR3, TCP20, TCPZ, TTCP20
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-101926 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCT6A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total CCT6A.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Chaperonin-containing tailless complex polypeptide 1 subunit 6A correlates with increased World Health Organization grade, less isocitrate dehydrogenase mutation, and deteriorative survival of astrocytoma patients.
MALDI-IMS combined with shotgun proteomics identify and localize new factors in male infertility.
Hu Y, Fu P, Zhao H, Zhang F, Jiang X, Zhao W, Lv P
Journal of clinical laboratory analysis 2021 Sep;35(9):e23917
Journal of clinical laboratory analysis 2021 Sep;35(9):e23917
MALDI-IMS combined with shotgun proteomics identify and localize new factors in male infertility.
Lahiri S, Aftab W, Walenta L, Strauss L, Poutanen M, Mayerhofer A, Imhof A
Life science alliance 2021 Mar;4(3)
Life science alliance 2021 Mar;4(3)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCT6A in HepG2 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with CCT6A polyclonal antibody (Product # PA5-101926).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CCT6A in HepG2 cell lysate (left lane: treated with the antigen-specific peptide). Samples were incubated with CCT6A polyclonal antibody (Product # PA5-101926).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CCT6A in HepG2 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CCT6A polyclonal antibody (Product # PA5-101926) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of paraffin-embedded CCT6A in human prostate tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CCT6A polyclonal antibody (Product # PA5-101926) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6. Validation of MLP score based identification. Distribution pattern of some key proteins in imaging mass spectrometry (IMS) measurements (as identified by MLP scoring) and their corresponding corroboration by immunohistochemical staining (IHC). (A) Interstitial distribution of Mimecan (Ogn) (upper panel: ion image of m/z = 763.5 as measured in IMS; lower panel: IHC, interstitial staining indicated by *). In both IMS and IHC, the protein is observed to be present only in AROM+. (B) Interstitial distribution of Collagen I (Cola1) (upper panel: ion image of m/z = 929.5 as measured in IMS; lower panel: IHC, interstitial staining indicated by *). In both IMS and IHC, the protein is observed to be highly up-regulated in AROM+. (C) Tubular distribution of T-complex protein 1 subunit zeta (Cct6a) (upper panel: ion image of m/z = 784.5 as measured in IMS; lower panel: IHC, tubular staining indicated by *). In both IMS and IHC, the protein is observed to be present only in WT.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1 FIGURE Chaperonin-containing tailless complex polypeptide 1 subunit 6A protein expression in astrocytoma patients. Diagram of different IHC grade of CCT6A expression in astrocytoma (X200; A). Distribution of IHC score of CCT6A in astrocytoma patients (B). CCT6A, chaperonin-containing tailless complex polypeptide 1 subunit 6A; IHC, immunohistochemistry
