H00003399-M02

antibody from Abnova Corporation

Targeting: ID3 bHLHb25, HEIR-1

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003399-M02

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003399-M02, RRID:AB_733185

Product name

ID3 monoclonal antibody (M02), clone 3E10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ID3.

Antigen sequence

MKALSPVRGCYEAVCCLSERSLAIARGRGKGPAAE
EPLSLLDDMNHCYSRLRELVPGVPRGTQLSQVEIL
QRVIDYILDLQVV


Isotype

IgG

Antibody clone number

3E10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Increased expression of Id1 and Id3 promotes tumorigenicity by enhancing angiogenesis and suppressing apoptosis in small cell lung cancer.

Chen D, Forootan SS, Gosney JR, Forootan FS, Ke Y
Genes & cancer 2014 May;5(5-6):212-25

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Increased expression of Id1 and Id3 promotes tumorigenicity by enhancing angiogenesis and suppressing apoptosis in small cell lung cancer.

Chen D, Forootan SS, Gosney JR, Forootan FS, Ke Y
Genes & cancer 2014 May;5(5-6):212-25

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ID3 is approximately 0.3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to ID3 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol