935301

antibody from BioLegend

Targeting: MYB c-myb

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

935301

Provider

BioLegend

Product name

Purified anti-c-Myb

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16209A

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg protein) from the indicated cell lines, A549 (negative control), RPMI 8226, U-937, THP-1, Jurkat, and MOLT-4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-c-Myb antibody (clone W16209A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50,000 dilution (lower). Lane M: Molecular weight marker

Conjugate

Unconjugated