A00729-1

antibody from Boster Biological Technology

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A00729-1

Provider

Boster Biological Technology

Product name

Anti-L1CAM Antibody Picoband™

Antibody type

Polyclonal

Description

Polyclonal antibody for L1CAM detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. L1CAM information: Subcellular Localization: Cell membrane.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Prognostic Value of β-Catenin and L1CAM Expressions in Type I Endometrial Carcinoma.

Manule Y, Miskad UA, Masadah R, Nelwan B, Cangara MH, Mardiati M
Asian Pacific journal of cancer prevention : APJCP 2023 Apr 1;24(4):1413-1417

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of L1CAM using anti-L1CAM antibody (A00729-1). L1CAM was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-L1CAM Antibody (A00729-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image