AF277

antibody from R&D Systems

Targeting: L1CAM CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF277

Provider

R&D Systems

Product name

Human L1CAM Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human L1CAM in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ICAM-2 and rhICAM-3 is observed and less than 1% cross-reactvity with rhCD31 and recombinant mouse VCAM-1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

CAA42508


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Secretome and degradome profiling shows that Kallikrein-related peptidases 4, 5, 6, and 7 induce TGFβ-1 signaling in ovarian cancer cells.

Shahinian H, Loessner D, Biniossek ML, Kizhakkedathu JN, Clements JA, Magdolen V, Schilling O
Molecular oncology 2014 Feb;8(1):68-82

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The L1 cell adhesion molecule is a potential biomarker of human distal nephron injury in acute tubular necrosis.

Allory Y, Audard V, Fontanges P, Ronco P, Debiec H
Kidney international 2008 Mar;73(6):751-8

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

L1CAM in Human Brain. L1CAM was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human L1CAM Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF277) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to plasma membranes and cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.