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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-32876 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIEZO1 Monoclonal Antibody (2-10)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10-Feb
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Three-Dimensionally Printed Ti2448 With Low Stiffness Enhanced Angiogenesis and Osteogenesis by Regulating Macrophage Polarization via Piezo1/YAP Signaling Axis.
Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans.
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction.
Tang Z, Wei X, Li T, Wu H, Xiao X, Hao Y, Li S, Hou W, Shi L, Li X, Guo Z
Frontiers in cell and developmental biology 2021;9:750948
Frontiers in cell and developmental biology 2021;9:750948
Shear-stress sensing by PIEZO1 regulates tendon stiffness in rodents and influences jumping performance in humans.
Passini FS, Jaeger PK, Saab AS, Hanlon S, Chittim NA, Arlt MJ, Ferrari KD, Haenni D, Caprara S, Bollhalder M, Niederöst B, Horvath AN, Götschi T, Ma S, Passini-Tall B, Fucentese SF, Blache U, Silván U, Weber B, Silbernagel KG, Snedeker JG
Nature biomedical engineering 2021 Dec;5(12):1457-1471
Nature biomedical engineering 2021 Dec;5(12):1457-1471
Adaptable pulsatile flow generated from stem cell-derived cardiomyocytes using quantitative imaging-based signal transduction.
Qian T, Gil DA, Contreras Guzman E, Gastfriend BD, Tweed KE, Palecek SP, Skala MC
Lab on a chip 2020 Oct 21;20(20):3744-3756
Lab on a chip 2020 Oct 21;20(20):3744-3756
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIEZO1 in recombinant protein using a PIEZO1 Monoclonal antibody (Product # MA5-32876) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIEZO1 in K562 cell lysates using a PIEZO1 Monoclonal antibody (Product # MA5-32876) at a dilution of 1:500. Secondary antibody was used at 1:40,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of PIEZO1 in A431 cells using a PIEZO1 Monoclonal antibody (Product # MA5-32876).Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of PIEZO1 of paraffin-embedded Mouse brain tissue using a PIEZO1 Monoclonal antibody (Product #MA5-32876). Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 12 Substrate stiffness regulates Piezo1 expression and YAP nuclear translocation. Piezo1 expression and cell adhesion to the extracellular matrix (focal adhesion) were observed by immunofluorescence. Scale bar = 20 um (A) . Effect of different substrate stiffnesses on YAP localization in macrophages evaluated by immunofluorescence staining. Scale bar = 20 um (B) . Piezo1 and YAP expression in different groups (C) . Quantitative analysis of immunofluorescence intensity (D,E) . Note: * p < 0.05 compared to the control; # p < 0.05 compared to Ti2448.
