600-401-672

antibody from Rockland Immunochemicals, Inc.

Targeting: FANCC FA3, FAC, FACC

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-672

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-672, RRID:AB_2101901

Product name

Anti-Human FANCC (RABBIT) Antibody - 600-401-672

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's affinity purified anti-FANCC antibody shows detection of a band at ~63 kDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate.  The identity of the lower molecular weight band is unknown.  Approximately 35 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,500 in PBS supplemented with 1% normal goat serum and 0.1% BLOTTO (B501-0500). The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers (indicated at left).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot