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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
Reference
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- Product number
- H00008505-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00008505-B01P, RRID:AB_1677774
- Product name
- PARG purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human PARG protein.
- Antigen sequence
MNAGPGCEPCTKRPRWGAATTSPAASDARSFPSRQ
RRVLDPKDAHVQFRVPPSSPACVPGRAGQHRGSAT
SLVFKQKTITSWMDTKGIKTAESESLDSKENNNTR
IESMMSSVQKDNFYQHNVEKLENVSQLSLDKSPTE
KSTQYLNQHQTAAMCKWQNEGKHTEQLLESEPQTV
TLVPEQFSNANIDRSPQNDDHSDTDSEENRDNQQF
LTTVKLANAKQTTEDEQAREAKSHQKCSKSCDPGE
DCASCQQDEIDVVPESPLSDVGSEDVGTGPKNDNK
LTRQESCLGNSPPFEKESEPESPMDVDNSKNSCQD
SEADEETSPGFDEQEDGSSSQTANKPSRFQARDAD
IEFRKRYSTKGGEVRLHFQFEGGESRTGMNDLNAK
LPGNISSLNVECRNSKQHGKKDSKITDHFMRLPKA
EDRRKEQWETKHQRTERKIPKYVPPHLSPDKKWLG
TPIEEMRRMPRCGIRLPLLRPSANHTVTIRVDLLR
AGEVPKPFPTHYKDLWDNKHVKMPCSEQNLYPVED
ENGERTAGSRWELIQTALLNKFTRPQNLKDAILKY
NVAYSKKWDFTALIDFWDKVLEEAEAQHLYQSILP
DMVKIALCLPNICTQPIPLLKQKMNHSITMSQEQI
ASLLANAFFCTFPRRNAKMKSEYSSYPDINFNRLF
EGRSSRKPEKLKTLFCYFRRVTEKKPTGLVTFTRQ
SLEDFPEWERCEKPLTRLHVTYEGTIEENGQGMLQ
VDFANRFVGGGVTSAGLVQEEIRFLINPELIISRL
FTEVLDHNECLIITGTEQYSEYTGYAETYRWSRSH
EDGSERDDWQRRCTEIVAIDALHFRRYLDQFVPEK
MRRELNKAYCGFLRPGVSSENLSAVATGNWGCGAF
GGDARLKALIQILAAAAAERDVVYFTFGDSELMRD
IYSMHIFLTERKLTVGDVYKLLLRYYNEECRNCST
PGPDIKLYPFIYHAVESCAETADHSGQRTGT- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references PARP-2 depletion results in lower radiation cell survival but cell line-specific differences in poly(ADP-ribose) levels.
Boudra MT, Bolin C, Chiker S, Fouquin A, Zaremba T, Vaslin L, Biard D, Cordelières FP, Mégnin-Chanet F, Favaudon V, Fernet M, Pennaneach V, Hall J
Cellular and molecular life sciences : CMLS 2015 Apr;72(8):1585-97
Cellular and molecular life sciences : CMLS 2015 Apr;72(8):1585-97
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PARG expression in transfected 293T cell line (H00008505-T01) by PARG MaxPab polyclonal antibody.Lane 1: PARG transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
- Validation comment
- Western Blot (Transfected lysate)
- Protocol
- Protocol
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PARG MaxPab polyclonal antibody. Western Blot analysis of PARG expression in Hela S3 NE.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PARG expression in transfected 293T cell line (H00008505-T01) by PARG MaxPab polyclonal antibody.Lane 1: PARG transfected lysate(107.36 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to PARG on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
