Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN359530 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-PARG (PARG) (C-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human Parg.
- Description
- Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store the antibody at 2 - 8°C up to one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Poly(ADP-ribose) polymerase 1 accelerates single-strand break repair in concert with poly(ADP-ribose) glycohydrolase.
Two small enzyme isoforms mediate mammalian mitochondrial poly(ADP-ribose) glycohydrolase (PARG) activity.
MNNG-induced cell death is controlled by interactions between PARP-1, poly(ADP-ribose) glycohydrolase, and XRCC1.
Fisher AE, Hochegger H, Takeda S, Caldecott KW
Molecular and cellular biology 2007 Aug;27(15):5597-605
Molecular and cellular biology 2007 Aug;27(15):5597-605
Two small enzyme isoforms mediate mammalian mitochondrial poly(ADP-ribose) glycohydrolase (PARG) activity.
Meyer RG, Meyer-Ficca ML, Whatcott CJ, Jacobson EL, Jacobson MK
Experimental cell research 2007 Aug 1;313(13):2920-36
Experimental cell research 2007 Aug 1;313(13):2920-36
MNNG-induced cell death is controlled by interactions between PARP-1, poly(ADP-ribose) glycohydrolase, and XRCC1.
Keil C, Gröbe T, Oei SL
The Journal of biological chemistry 2006 Nov 10;281(45):34394-405
The Journal of biological chemistry 2006 Nov 10;281(45):34394-405
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Parg (arrow) using rabbit polyclonal Parg Antibody (C-term) (AP14014PU-N). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the Parg gene (Lane 2) (Origene Technologies).
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of Parg Antibody (C-term) in 293 cell line lysates (35 μg/lane). Parg (arrow) was detected using the purified Pab (1:60 dilution).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Parg antibody (C-term) (AP14014PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.