PAB28629

antibody from Abnova Corporation

Targeting: DLST DLTS, KGD2, OGDC-E2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28629

Provider

Abnova Corporation

Product name

DLST polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant DLST.

Antigen sequence

QNTCAMLTTFNEIDMSNIQEMRARHKEAFLKKHNL
KLGFMSAFVKASAFALQEQPVVNAVIDDTTKEVVY
RDYIDISVAVATPRGLVVPVIRNVEAMNFADIERT
ITELGEKARKNELAIEDMDGGTFTISNGGVFGSLF
GTPII


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Human cell line RT-4, Lane 2: Human cell line U-251MG sp, Lane 3: Human cell line A-431, Lane 4: Human liver tissue with DLST polyclonal antibody (Cat # PAB28629).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells), Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla) with DLST polyclonal antibody (Cat # PAB28629).

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with DLST polyclonal antibody (Cat # PAB28629) at 1-4 ug/ml shows positivity in nucleus but not nucleoli, cytoplasm & mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human liver with DLST polyclonal antibody (Cat # PAB28629) shows strong cytoplasmic positivity in hepatocytes at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)