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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [3]
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- Product number
- GTX629478 - Provider product page
- Provider
- GeneTex
- Product name
- HMGA2 antibody [GT763]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- HMGA2 antibody [GT763] detects HMGA2 protein by western blot analysis.A. 30 ?g HepG2 whole cell lysate/extract15% SDS-PAGEHMGA2 antibody [GT763] (GTX629478) dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody [GT763] (GTX629478) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody [GT763] detects HMGA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody [GT763] (GTX629478) diluted at 1:200.Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 £gg of HMGA2 antibody [GT763] (GTX629478).Western blot analysis was performed using HMGA2 antibody [GT763] (GTX629478).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody [GT763] detects HMGA2 protein at on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody [GT763] detects HMGA2 protein at on SkHep1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SkHep1 xenograft. HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HMGA2 antibody [GT763] detects HMGA2 protein on C2C12 by immunohistochemical analysis. Sample: Paraffin-embedded C2C12. HMGA2 antibody [GT763] (GTX629478) dilution: 1:200.
