GTX100519

antibody from GeneTex

Targeting: HMGA2 BABL, HMGIC, LIPO

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX100519

Provider

GeneTex

Proper citation

GeneTex Cat#GTX100519, RRID:AB_1950498

Product name

HMGA2 antibody - ChIP grade

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Tetrac downregulates β-catenin and HMGA2 to promote the effect of resveratrol in colon cancer.

Nana AW, Chin YT, Lin CY, Ho Y, Bennett JA, Shih YJ, Chen YR, Changou CA, Pedersen JZ, Incerpi S, Liu LF, Whang-Peng J, Fu E, Li WS, Mousa SA, Lin HY, Davis PJ
Endocrine-related cancer 2018 Mar;25(3):279-293

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The long noncoding RNA HULC promotes liver cancer by increasing the expression of the HMGA2 oncogene via sequestration of the microRNA-186.

Wang Y, Chen F, Zhao M, Yang Z, Li J, Zhang S, Zhang W, Ye L, Zhang X
The Journal of biological chemistry 2017 Sep 15;292(37):15395-15407

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Effect of RNA interference of the expression of HMGA2 on the proliferation and invasion ability of ACHN renal cell carcinoma cells.

Liu Y, Fu QZ, Pu L, Song LL, Wang YY, Liu J, Wang ZL, Wang ZM
Molecular medicine reports 2017 Oct;16(4):5107-5112

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HMGA2 promotes vasculogenic mimicry and tumor aggressiveness by upregulating Twist1 in gastric carcinoma.

Sun J, Sun B, Sun R, Zhu D, Zhao X, Zhang Y, Dong X, Che N, Li J, Liu F, Zhao N, Wang Y, Zhang D
Scientific reports 2017 May 22;7(1):2229

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The Fragment HMGA2-sh-3p20 from HMGA2 mRNA 3'UTR Promotes the Growth of Hepatoma Cells by Upregulating HMGA2.

Wang Y, Chen F, Yang Z, Zhao M, Zhang S, Gao Y, Feng J, Yang G, Zhang W, Ye L, Zhang X
Scientific reports 2017 May 18;7(1):2070

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Tough decoy targeting of predominant let-7 miRNA species in adult human hematopoietic cells.

de Vasconcellos JF, Byrnes C, Lee YT, Allwardt JM, Kaushal M, Rabel A, Miller JL
Journal of translational medicine 2017 Aug 2;15(1):169

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MiR-107 suppresses proliferation of hepatoma cells through targeting HMGA2 mRNA 3'UTR.

Wang Y, Chen F, Zhao M, Yang Z, Zhang S, Ye L, Gao H, Zhang X
Biochemical and biophysical research communications 2016 Nov 18;480(3):455-460

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The ubiquitin ligase human TRIM71 regulates let-7 microRNA biogenesis via modulation of Lin28B protein.

Lee SH, Cho S, Kim MS, Choi K, Cho JY, Gwak HS, Kim YJ, Yoo H, Lee SH, Park JB, Kim JH
Biochimica et biophysica acta 2014 May;1839(5):374-86

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

KD Reagent Type

siRNA

Downregulation

<50%

KD Reagent Provider

shRNA reagents was provided courtesy of cooperative labs.

KD Reagent Prod no

Proprietary.

KD Reagent Prod page

KD Reagent Prod page

Antibody Lot Number

42123

Show more

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 15% SDS PAGE GTX100519 diluted at 1:10000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 15 % SDS-PAGE, and blotted with HMGA2 antibody (GTX100519) diluted by 1:10000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted by 1:10000.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody (GTX100519) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: HMGA2 protein stained by HMGA2 antibody (GTX100519) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 £gg of HMGA2 antibody (GTX100519).Western blot analysis was performed using HMGA2 antibody (GTX100519).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2(GTX100519) antibody at 1:500 dilution.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human HuCCT1 xenograft . HMGA2 antibody - ChIP grade (GTX100519) diluted at 1:250.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HMGA2 antibody (GTX100519) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. HMGA2 antibody (GTX100519) diluted at 1:500.