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antibody from Invitrogen Antibodies
Targeting: HMGA2
BABL, HMGIC, LIPO
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Chromatin Immunoprecipitation Other assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
- Other assay [1]
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- Product number
- PA5-21320 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HMGA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, HeLa, HepG2, HCT116. Predicted reactivity: Mouse (95%), Rat (92%), Zebrafish (85%), Xenopus laevis (80%), Chicken (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.08 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references HMGA2 regulates circular RNA ASPH to promote tumor growth in lung adenocarcinoma.
Xu L, Ma Y, Zhang H, Lu QJ, Yang L, Jiang GN, Liao WL
Cell death & disease 2020 Jul 27;11(7):593
Cell death & disease 2020 Jul 27;11(7):593
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGA2 using 30 µg of A) HepG2 and B) HCT116 lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a HMGA2 polyclonal antibody (Product # PA5-21320) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HMGA2 using Whole cell extracts (30 µg). Samples were loaded onto a 15% SDS-PAGE gel and probed with a HMGA2 polyclonal antibody (Product # PA5-21320) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HMGA2 was performed by separating 30 µg of whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMGA2 Polyclonal Antibody (Product # PA5-21320) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HMGA2 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMGA2 Polyclonal Antibody (Product # PA5-21320) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of HMGA2 was performed by separating 30 µg of various whole cell extracts by 15% SDS-PAGE. Proteins were transferred to a membrane and probed with a HMGA2 Polyclonal Antibody (Product # PA5-21320) at a dilution of 1:10000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody (Product # PA5-21320) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HMGA2 was performed in paraffin-embedded human HuCCT1 xenograft tissue using HMGA2 Polyclonal Antibody (Product # PA5-21320) at a dilution of 1:250.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HMGA2 antibody (Product # PA5-21320) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. HMGA2 antibody (Product # PA5-21320) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2 (Product # PA5-21320) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2 (Product # PA5-21320) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 The oncogenic effects of circASPH in vitro and in vivo. a The morphologic changes in A549 and PC9 cells overexpressing circASPH were observed using a scanning electron microscopy assay. b , c DNA synthesis assessed using an EdU assay in the lenti-circASPH cells and control cells. Nuclei were stained with Hoechst 33342 (blue). The quantified data are presented as the percentage of EdU-incorporated cells. Log2 transformation was applied to deal with the ratio results and to obtain the normally distributed data. Then, T test was used to compare the difference between groups. d , e The migration of A549 and PC9 cells was significantly increased by circASPH overexpression. The migration distances (mum) of A549 and PC9 cells were quantified. f , g The invasion of A549 and PC9 cells was apparently increased by circASPH overexpression. The quantified data are presented as the number of invading cells per HPF. HPF high-power field. h - j Xenograft tumor models show that tumors grown from the circASPH-overexpressing cells were bigger than those grown from the control cells. k Western blot analysis of the levels of HMGA2 protein in the xenograft tumors. l IHC staining of the HMGA2 protein in the xenograft tumors. Every cell test was carried out in triplicate, and n = 3 for each cell group.
