AP07808SU-N

antibody from Acris Antibodies GmbH

Targeting: ANGPT1 Ang1, KIAA0003

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP07808SU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP07808SU-N, RRID:AB_1611528

Product name

anti Angiopoietin-1 (N-term)

Antibody type

Polyclonal

Antigen

Synthetic Peptide corresponding to a region (aa 21-40) near the N-terminus of Mouse angiopoietin 1 protein conjugated to KLH using maleimide. Epitope: N-terminus.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

50 µl

Concentration

85 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot against supernatants of Mouse Angiopoietin-expressing endothelial cells using Angiopoietin-1 antibody Cat.-No AP07808SU-N at a 1/500 dilution to detect Mouse Ang-1. Lane 1: Wt endothelial cells. Lane 2: Mouse Ang-1 (clone 1-8) expressing cells. Lane 3: Mouse Ang-1 (clone 1-15) expressing cells.Lane 4: Mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1/5000 dilution of HRP conjugated Goat anti Rabbit IgG preceded color development.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded Human Lung Vessel stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded Mouse Liver stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded Mouse Kidney stained with Angiopoietin-1 antibody Cat.-No AP07808SU-N after heat-induced antigen retrieval.