MAB923-100

antibody from R&D Systems

Targeting: ANGPT1 Ang1, KIAA0003

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB923-100

Provider

R&D Systems

Product name

Human Angiopoietin-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Angiopoietin-2, recombinant mouse Angiopoietin-like 3, rhAngiopoietin-4, or rhAngiopoietin-like 7 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

Q15389


Isotype

IgG

Antibody clone number

171718

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Pulmonary pericytes regulate lung morphogenesis.

Kato K, Diéguez-Hurtado R, Park DY, Hong SP, Kato-Azuma S, Adams S, Stehling M, Trappmann B, Wrana JL, Koh GY, Adams RH
Nature communications 2018 Jun 22;9(1):2448

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Fibulin-5 Regulates Angiopoietin-1/Tie-2 Receptor Signaling in Endothelial Cells.

Chan W, Ismail H, Mayaki D, Sanchez V, Tiedemann K, Davis EC, Hussain SN
PloS one 2016;11(6):e0156994

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Early systemic microvascular damage in pigs with atherogenic diabetes mellitus coincides with renal angiopoietin dysbalance.

Khairoun M, van den Heuvel M, van den Berg BM, Sorop O, de Boer R, van Ditzhuijzen NS, Bajema IM, Baelde HJ, Zandbergen M, Duncker DJ, Rabelink TJ, Reinders ME, van der Giessen WJ, Rotmans JI
PloS one 2015;10(4):e0121555

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High bone marrow angiopoietin-1 expression is an independent poor prognostic factor for survival in patients with myelodysplastic syndromes.

Cheng CL, Hou HA, Jhuang JY, Lin CW, Chen CY, Tang JL, Chou WC, Tseng MH, Yao M, Huang SY, Ko BS, Hsu SC, Wu SJ, Tsay W, Chen YC, Tien HF
British journal of cancer 2011 Sep 27;105(7):975-82

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Regulation of angiopoietin expression by bacterial lipopolysaccharide.

Mofarrahi M, Nouh T, Qureshi S, Guillot L, Mayaki D, Hussain SN
American journal of physiology. Lung cellular and molecular physiology 2008 May;294(5):L955-63

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Angiopoietin 1 directly induces destruction of the rheumatoid joint by cooperative, but independent, signaling via ERK/MAPK and phosphatidylinositol 3-kinase/Akt.

Hashiramoto A, Sakai C, Yoshida K, Tsumiyama K, Miura Y, Shiozawa K, Nose M, Komai K, Shiozawa S
Arthritis and rheumatism 2007 Jul;56(7):2170-9

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Angiopoietin-1 in Human Prostate Cancer Tissue. Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human Angiopoietin-1 Monoclonal Antibody (Catalog # MAB923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.