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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-32150 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Angiopoietin 1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is specific for mouse Angiopoietin 1 (mAng-1). Partial cross-reactivity is noted with human Angiopoietin 2. Store as a concentrated solution. Centrifuge briefly prior to opening vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 80 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Efficiency of Gymnema sylvestre-derived gymnemic acid on the restoration and improvement of brain vascular characteristics in diabetic rats.
Sandech N, Jangchart R, Komolkriengkrai M, Boonyoung P, Khimmaktong W
Experimental and therapeutic medicine 2021 Dec;22(6):1420
Experimental and therapeutic medicine 2021 Dec;22(6):1420
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Angiopoietin 1 was performed by separating 20 µg of various supernatants of mouse angiopoietin-expressing endothelial cells by 10% SDS-PAGE. Angiopoietin 1 Polyclonal Antibody (Product # PA1-32150) was used as the primary antibody. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Angiopoietin 1 in human lung tissue using Angiopoietin 1 Polyclonal Antibody (Product # PA1-32150) at a dilution of 1:500. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 11 Densitometric analysis of VEGF and Ang-1 protein expression in the hypothalamus of control, CGM, STZ, STZ + GM, and STZ + GL rats at 8 weeks. Data are presented as the mean +- SEM. * P
