Explore
Validate
Learn
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF923 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse Angiopoietin-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human and mouse Angiopoietin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) Angiopoietin-2 and rhAngiopoietin-4 is observed.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q5HYA0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references The Fibrin Cleavage Product Bβ(15-42) Channels Endothelial and Tubular Regeneration in the Post-acute Course During Murine Renal Ischemia Reperfusion Injury.
Ang-2 but not Ang-1 expression in perivascular soft tissue tumors.
Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers.
Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Fischer D, Seifen C, Baer P, Jung M, Mertens C, Scheller B, Zacharowski K, Hofmann R, Maier TJ, Urbschat A
Frontiers in pharmacology 2018;9:369
Frontiers in pharmacology 2018;9:369
Ang-2 but not Ang-1 expression in perivascular soft tissue tumors.
Shrestha S, Shen J, Giacomelli P, Scott MA, Soo C, Ting K, Péault B, Dry SM, James AW
Journal of orthopaedics 2017 Mar;14(1):147-153
Journal of orthopaedics 2017 Mar;14(1):147-153
Impact of p16 status on pro- and anti-angiogenesis factors in head and neck cancers.
Baruah P, Lee M, Wilson PO, Odutoye T, Williamson P, Hyde N, Kaski JC, Dumitriu IE
British journal of cancer 2015 Aug 11;113(4):653-9
British journal of cancer 2015 Aug 11;113(4):653-9
Angiopoietin-4 promotes glioblastoma progression by enhancing tumor cell viability and angiogenesis.
Brunckhorst MK, Wang H, Lu R, Yu Q
Cancer research 2010 Sep 15;70(18):7283-93
Cancer research 2010 Sep 15;70(18):7283-93
Integrin binding angiopoietin-1 monomers reduce cardiac hypertrophy.
Dallabrida SM, Ismail NS, Pravda EA, Parodi EM, Dickie R, Durand EM, Lai J, Cassiola F, Rogers RA, Rupnick MA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Aug;22(8):3010-23
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Aug;22(8):3010-23
Angiogenesis markers (VEGF, soluble receptor of VEGF and angiopoietin-1) in very early arthritis and their association with inflammation and joint destruction.
Clavel G, Bessis N, Lemeiter D, Fardellone P, Mejjad O, Ménard JF, Pouplin S, Boumier P, Vittecoq O, Le Loët X, Boissier MC
Clinical immunology (Orlando, Fla.) 2007 Aug;124(2):158-64
Clinical immunology (Orlando, Fla.) 2007 Aug;124(2):158-64
Enhancement of angiogenic effectors through hypoxia-inducible factor in preterm primate lung in vivo.
Asikainen TM, Waleh NS, Schneider BK, Clyman RI, White CW
American journal of physiology. Lung cellular and molecular physiology 2006 Oct;291(4):L588-95
American journal of physiology. Lung cellular and molecular physiology 2006 Oct;291(4):L588-95
Activation of hypoxia-inducible factors in hyperoxia through prolyl 4-hydroxylase blockade in cells and explants of primate lung.
Asikainen TM, Schneider BK, Waleh NS, Clyman RI, Ho WB, Flippin LA, Günzler V, White CW
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
Proceedings of the National Academy of Sciences of the United States of America 2005 Jul 19;102(29):10212-7
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-1 in Human Prostate Cancer Tissue. Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using 15 µg/mL Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-1 in Human Prostate. Angiopoietin-1 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Angiopoietin-1 in Mouse Embryo. Angiopoietin-1 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Human/Mouse Angiopoietin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF923) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to endothelium in the loop of the midgut. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
