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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-112439 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- H2A.ZK11ac Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.12 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Acetyl-H2A.ZK11ac (Lys11) using a polyclonal antibody (Product # PA5-112439) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in HepG2 whole cell lysate (treated with 30 mM sodium butyrate for 4 hours). Observed band: 14 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Acetyl-H2A.ZK11ac (Lys11) in Hela cells using a Acetyl-H2A.ZK11ac (Lys11) polyclonal antibody (Product # PA5-112439) at a dilution of 1:100. The cells were treated with 30mM sodium butyrate for 4 hours. The cells were then fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the primary antibody overnight at 4°C. The secondary antibody was an Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation analysis of Acetyl-H2A.ZK11ac (Lys11) in 4*10^6 Hela cells using a Acetyl-H2A.ZK11ac (Lys11) polyclonal antibody (Product #PA5-112439). Hela cells were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8 µg anti-H2AFZ or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta-Globin promoter.
