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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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Validation data
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- Product number
- 16481-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PHAX Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MALEVGDME DGQLSDSDSD MTVAPSDRPL QLPKVLGGDS AMRAFQNTAT ACAPVSHYRA VESVDSSEES FSDSDDDSCL WKRKRQKCFN PPPKPEPFQF GQSSQKPPVA GGKKINNIWG AVLQEQNQDA VATELGILGM EGTIDRSRQS ETYNYLLAKK LRKESQEHTK DLDKELDEYM HGGKKMGSKE EENGQGHLKR KRPVKDRLGN RPEMNYKGRY EITAEDSQEK VADEISFRLQ EPKKDLIARV VRIIGNKKAI ELLMETAEVE QNGGLFIMNG SRRRTPGGVF LNLLKNTPSI SEEQIKDIFY IENQKEYENK KAARKRRTQV LGKKMKQAIK SLNFQEDDDT SRETFASDTN EALASLDESQ EGHAEAKLEA EEAIEVDHSH DLDIF (1-394 aa encoded by BC021161)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.15 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16481-1-AP (PHAX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16481-1-AP (PHAX antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
