LS-C407972

antibody from LSBio

Targeting: FABP3 FABP11, H-FABP, MDGI, O-FABP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C407972

Provider

LSBio

Product name

FABP3 / H-FABP Antibody (aa15-48) LS-C407972

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human, Mouse, Rat, Hamster

Host

Rabbit

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cardiac FABP antibody Western blot. All lanes: Anti Cardiac FABP at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Predicted band size: 20 kD. Observed band size: 20 kD.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cardiac FABP antibody IHC-paraffin. IHC(P): Mouse Cardiac Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cardiac FABP antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cardiac FABP antibody IHC-paraffin. IHC(P): Mouse Cardiac Muscle Tissue.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cardiac FABP antibody IHC-paraffin. IHC(P): Rat Kidney Tissue.

Blocking/Neutralizing

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis of Cardiac FABP using anti-Cardiac FABP antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse heart tissue lysate, Lane 2: mouse heart tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cardiac FABP antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cardiac FABP at approximately 15KD. The expected band size for Cardiac FABP is at 15KD.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Cardiac FABP antibody Western blot. All lanes: Anti Cardiac FABP at 0.5 ug/ml. Lane 1: Rat Liver Tissue Lysate at 50 ug. Lane 2: Rat Kidney Tissue Lysate at 50 ug. Predicted band size: 20 kD. Observed band size: 20 kD.