AF1678

antibody from R&D Systems

Targeting: FABP3 FABP11, H-FABP, MDGI, O-FABP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1678

Provider

R&D Systems

Product name

Human/Mouse/Rat FABP3/H-FABP Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human FABP3/H-FABP in direct ELISAs and detects human, mouse, and rat FABP3/H-FABP in Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse (rm) FABP4 and recombinant human (rh) FABP4 is observed, approximately 15% cross-reactivity with recombinant rat (rr) FABP1, rhFABP1, rhFABP2, rhFABP7, and rhFABP8 is observed, and less than 10% cross-reactivity with rrFABP2 and rmFABP5 is observed.

Reactivity

Human, Mouse, Rat

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

P05413


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Expression of fatty acid-binding proteins in adult hippocampal neurogenic niche of postischemic monkeys.

Boneva NB, Kaplamadzhiev DB, Sahara S, Kikuchi H, Pyko IV, Kikuchi M, Tonchev AB, Yamashima T
Hippocampus 2011 Feb;21(2):162-71

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Formation of a human-derived fat tissue layer in P(DL)LGA hollow fibre scaffolds for adipocyte tissue engineering.

Morgan SM, Ainsworth BJ, Kanczler JM, Babister JC, Chaudhuri JB, Oreffo RO
Biomaterials 2009 Apr;30(10):1910-7

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The use of small interfering RNAs to inhibit adipocyte differentiation in human preadipocytes and fetal-femur-derived mesenchymal cells.

Xu Y, Mirmalek-Sani SH, Yang X, Zhang J, Oreffo RO
Experimental cell research 2006 Jun 10;312(10):1856-64

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human, Mouse, and Rat FABP3/H-FABP by Western Blot. Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse FABP3/H-FABP by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Sheep Anti-Human FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

FABP3/H-FABP in Human Heart. FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Sheep Anti-Human/Mouse/Rat FABP3/H-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.