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LearnPA5-71455
antibody from Invitrogen Antibodies
Targeting: FOXM1
FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP2, TGT3, trident
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-71455 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FOXM1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Dog: 83%; Guinea Pig: 83%; Human: 100%; Mouse: 91%; Rabbit: 91%; Rat: 91%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Mydgf promotes Cardiomyocyte proliferation and Neonatal Heart regeneration.
FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.
Wang Y, Li Y, Feng J, Liu W, Li Y, Liu J, Yin Q, Lian H, Liu L, Nie Y
Theranostics 2020;10(20):9100-9112
Theranostics 2020;10(20):9100-9112
FZD5 contributes to TNBC proliferation, DNA damage repair and stemness.
Sun Y, Wang Z, Na L, Dong D, Wang W, Zhao C
Cell death & disease 2020 Dec 12;11(12):1060
Cell death & disease 2020 Dec 12;11(12):1060
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXM1 on human pancreatic cancer cells, MDA-MB-231 and Huh-7 cells using 25 µg of sample per lane (Lane 1: MIA PaCa-2 human pancreatic cancer cells, 2: MDA-MB-231 cell lysate, 3: Huh-7 cell lysate). The sample was probed with a FOXM1 polyclonal antibody (Product # PA5-71455) using a primary antibody dilution of 1:2000 and a secondary antibody dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXM1 on human Jurkat cells. The sample was probed with a FOXM1 polyclonal antibody (Product # PA5-71455) using a primary antibody dilution of 1.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FOXM1 on human 786-0 cells. The sample was probed with a FOXM1 polyclonal antibody (Product # PA5-71455) using a primary antibody dilution of 1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on spleen cell tissue using a FOXM1 polyclonal antibody (Product # PA5-71455) with a dilution of 5.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on spleen cell tissue using a FOXM1 polyclonal antibody (Product # PA5-71455) with a dilution of 5.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 FOXM1 acts as a downstream effecter of FZD5. A CCLE database was interrogated for FZD5, FOXM1, BRCA1, and BIRC5 expression. Correlation between two genes in a total of 57 breast cancer cell lines was analyzed by Pearson statistics. B FOXM1, BRCA1, and BIRC5 expression in MDA-MB-231 cells was detected by Western blot. C FOXM1, BRCA1, and BIRC5 expression in Hs-578t cells was detected by Western blot. D Binding sites for FOXM1 on BRCA1 and BIRC5 promoters were shown. E Binding of FOXM1 to BRCA1 and BIRC5 promoters in MDA-MB-231 cells was analyzed by ChIP and real-time PCR, mean +- SD, n = 3. ** P < 0.01, *** P < 0.001, vs. IgG. NC negative control. F FOXM1, BRCA1, and BIRC5 expression in Hs-578t cells was detected by Western blot. G Active beta-catenin expression in MDA-MB-231 cells was detected by Western blot. H FOXM1 expression in MDA-MB-231 cells was detected by Western blot.
