PA5-117874

antibody from Invitrogen Antibodies

Targeting: CLNS1A CLCI, ICln

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117874

Provider

Invitrogen Antibodies

Product name

CLNS1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CLNS1A Polyclonal Antibody (Product # PA5-117874) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: K562 Whole Cell Lysate, Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 38 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using CLNS1A Polyclonal Antibody (Product # PA5-117874) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate, Lane B: HeLa Whole Cell Lysate, Lane C: K562 Whole Cell Lysate, Lane D: HepG2 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 38 kDa.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CLNS1A Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with CLNS1A Polyclonal Antibody (Product # PA5-117874) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: CLNS1A Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 26 kDa. Observed band size: 40 kDa.