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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS3549 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3549, RRID:AB_1661963
- Product name
- EGF (R709) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 681-730 of Human EGF.
- Description
- Epidermal growth factor (EGF) is an acid- and heat-stable 53 amino acid protein of 6 kDa originally found in rodents and humans. EGF has been shown to be a potent mitogen for a variety of cell types both in vivo and in vitro. It binds to the 170 kDa EGF receptor on the surface of cells and mediates intrinsic phos phoryl ation of the receptor on tyrosine residues. EGF has been detected in nearly all body fluids such as urine (urogastrone), saliva, milk and platelet-rich plasma.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of EGF (R709) pAb at 1:500 dilutionLane1:sp2/0 whole cell lysateLane2:Raw264.7 whole cell lysateLane3:PC12 whole cell lysate
