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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB236-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EGF Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human EGF in direct ELISAs. Detects human EGF and rat EGF in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) HB-EGF or rhTGF-alpha is observed. In Western blots, no cross-reactivity with recombinant mouse EGF is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 10825
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression and clinical value of EGFR in human meningiomas.
Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells.
Case report of multiple pustules of the bilateral lower limbs caused by a granulocyte colony-stimulating factor-producing solid pseudopapillary tumour of the pancreas.
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
S100A11, an dual mediator for growth regulation of human keratinocytes.
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Arnli MB, Backer-Grøndahl T, Ytterhus B, Granli US, Lydersen S, Gulati S, Torp SH
PeerJ 2017;5:e3140
PeerJ 2017;5:e3140
Epidermal growth factor as a mechanosensitizer in human bone marrow stromal cells.
Müller-Deubert S, Seefried L, Krug M, Jakob F, Ebert R
Stem cell research 2017 Oct;24:69-76
Stem cell research 2017 Oct;24:69-76
Case report of multiple pustules of the bilateral lower limbs caused by a granulocyte colony-stimulating factor-producing solid pseudopapillary tumour of the pancreas.
Iwata Y, Kobayashi T, Kuroda M, Mizoguchi Y, Arima M, Numata S, Watanabe S, Yagami A, Matsunaga K, Sugiura K
The British journal of dermatology 2017 Oct;177(4):1122-1126
The British journal of dermatology 2017 Oct;177(4):1122-1126
Anti-inflammatory activity of nanocrystalline silver-derived solutions in porcine contact dermatitis.
Nadworny PL, Wang J, Tredget EE, Burrell RE
Journal of inflammation (London, England) 2010 Feb 19;7:13
Journal of inflammation (London, England) 2010 Feb 19;7:13
S100A11, an dual mediator for growth regulation of human keratinocytes.
Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
Molecular biology of the cell 2008 Jan;19(1):78-85
Molecular biology of the cell 2008 Jan;19(1):78-85
Transforming growth factor-alpha: a major human serum factor that promotes human keratinocyte migration.
Li Y, Fan J, Chen M, Li W, Woodley DT
The Journal of investigative dermatology 2006 Sep;126(9):2096-105
The Journal of investigative dermatology 2006 Sep;126(9):2096-105
Salivary leptin induces increased expression of growth factors in oral keratinocytes.
Gröschl M, Topf HG, Kratzsch J, Dötsch J, Rascher W, Rauh M
Journal of molecular endocrinology 2005 Apr;34(2):353-66
Journal of molecular endocrinology 2005 Apr;34(2):353-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human and Rat EGF by Western Blot. Western blot shows 100 ng of Recombinant Human EGF (Catalog # 236-EG), Recombinant Mouse EGF (Catalog # 2028-EG) and Recombinant Rat EGF (Catalog # 3214-EG). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for EGF at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EGF in Human Skin. EGF was detected in immersion fixed paraffin-embedded sections of human skin using 25 µg/mL Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of keratinocytes in the stratum granulosum in epidermis. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by EGF and Neutralization by Human EGF Antibody. Recombinant Human EGF (Catalog # 236-EG) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human EGF (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human EGF Monoclonal Antibody (Catalog # MAB236). The ND50 is typically 0.05-0.1 µg/mL.
