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Western blot
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- References [2]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NBP2-32104 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TET2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Epigenetic Reprogramming Strategies to Reverse Global Loss of 5-Hydroxymethylcytosine, a Prognostic Factor for Poor Survival in High-grade Serous Ovarian Cancer.
Silencing HIF-1α induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells.
Tucker DW, Getchell CR, McCarthy ET, Ohman AW, Sasamoto N, Xu S, Ko JY, Gupta M, Shafrir A, Medina JE, Lee JJ, MacDonald LA, Malik A, Hasselblatt KT, Li W, Zhang H, Kaplan SJ, Murphy GF, Hirsch MS, Liu JF, Matulonis UA, Terry KL, Lian CG, Dinulescu DM
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 15;24(6):1389-1401
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 15;24(6):1389-1401
Silencing HIF-1α induces TET2 expression and augments ascorbic acid induced 5-hydroxymethylation of DNA in human metastatic melanoma cells.
Fischer AP, Miles SL
Biochemical and biophysical research communications 2017 Aug 19;490(2):176-181
Biochemical and biophysical research communications 2017 Aug 19;490(2):176-181
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-TET2 antibody NBP2-32104 used for IP at 6 ug/mg lysate. TET2 was also immunoprecipitated by rabbit anti-TET2 antibody BL16766. For blotting immunoprecipitated TET2, NBP2-32104 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TET2 Antibody [NBP2-32104] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-TET2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TET2 Antibody [NBP2-32104] - Human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TET2 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB
