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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [2]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX124204 - Provider product page
- Provider
- GeneTex
- Product name
- TET2 antibody [N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- TET2 antibody [N2], N-term detects TET2 protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extractB. 30 µg whole cell lysate/extract of human TET2-transfected 293T cells5 % SDS-PAGETET2 antibody [N2], N-term (GTX124204) dilution: 1:5000
- Validation comment
- WB
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- GeneTex (provider)
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- Experimental details
- TET2 antibody detects TET2 protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g whole cell lysate/extract of DDDDK-human TET2-transfected 293T cells5% SDS-PAGETET2 antibody (GTX124204) dilution: 1:5000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TET2 antibody [N2], N-term detects TET2 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with TET2 antibody [N2], N-term (GTX124204) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- TET2 antibody [N2], N-term detects TET2 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TET2 protein stained by TET2 antibody [N2], N-term (GTX124204) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar = 10 £gm.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- TET2 antibody [N2], N-term immunoprecipitates TET2 protein in IP experiments.IP samples: 30 £gg whole cell extract of TET2-transfected 293T cells.A. Control with 3 £gg of preimmune Rabbit IgGB. Immunoprecipitation of TET2 protein by 3 £gg TET2 antibody [N2], N-term (GTX124204)5 % SDS-PAGEThe immunoprecipitated TET2 protein was detected by TET2 antibody [N2], N-term (GTX124204) diluted at 1:3000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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- GeneTex (provider)
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- Experimental details
- Immunoprecipitation of TET2 protein from 293T whole cell extracts using 5 ?g of TET2 antibody [N2], N-term (GTX124204).Western blot analysis was performed using TET2 antibody [N2], N-term (GTX124204).EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.
Supportive validation
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- GeneTex (provider)
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- Experimental details
- Cross-linked ChIP was performed with U2OS chromatin extract and 5 £gg of either control rabbit IgG or anti-TET2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
