Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- MAB6055 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LRRC32/GARP Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LRRC32/GARP in ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse LRRC32 is observed.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
Q14392
- Isotype
- IgG
- Antibody clone number
- 855151
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Regulatory CD4 T cells inhibit HIV-1 expression of other CD4 T cell subsets via interactions with cell surface regulatory proteins.
Zhang M, Robinson TO, Duverger A, Kutsch O, Heath SL, Cron RQ
Virology 2018 Mar;516:21-29
Virology 2018 Mar;516:21-29
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of LRRC32/GARP in Human PBMCs stimulated to induce Tregs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) stimulated to induce Regulatory T Cells (Tregs) with 10 μg/mL Anti-CD3, 5 μg/mL Anti-CD28, 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B), and 20 ng/mLRecombinant Human IL-2 (Catalog # 202-IL) for 48 hours were stained with Mouse Anti-Human/Mouse LAP TGF-beta 1 APC-conjugated Monoclonal Antibody (Catalog # FAB2463A) and either (A) Mouse Anti-Human LRRC32/GARP Monoclonal Antibody (Catalog # MAB6055) or (B) Rat IgG1 Isotype Control (Catalog # MAB005) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).