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LearnNBP2-22126
antibody from Novus Biologicals
Targeting: SHH
HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-22126 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Sonic Hedgehog/Shh Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified.
- Reactivity
- Human, Mouse, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The sonic hedgehog signaling pathway contributes to the development of salivary gland neoplasms regardless of perineural infiltration.
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation.
Vidal MT, Lourenço SV, Soares FA, Gurgel CA, Studart EJ, Valverde Lde F, Araújo IB, Ramos EA, Xavier FC, Dos Santos JN
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9587-601
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9587-601
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation.
Valverde Lde F, Pereira Tde A, Dias RB, Guimarães VS, Ramos EA, Santos JN, Gurgel Rocha CA
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9233-41
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2016 Jul;37(7):9233-41
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis using Sonic Hedgehog mAb against HEK293 (1) and Sonic Hedgehog (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis using Sonic Hedgehog mAb against human Sonic Hedgehog recombinant protein. (Expected MW is 41 kDa)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Sonic Hedgehog Antibody (5H4) [NBP2-22126] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Sonic Hedgehog/Shh Antibody (5H4) [NBP2-22126] - Analysis of HeLa cells using Sonic Hedgehog mouse mAb (green) and negative control (red).
