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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA336923 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal Sonic Hedgehog/Shh Antibody (5H4)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal Sonic Hedgehog/Shh Antibody (5H4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- SHH
- Isotype
- IgG
- Antibody clone number
- 5H4
- Vial size
- 100 µl
- Concentration
- This product is unpurified. The exact concent
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Sonic Hedgehog Antibody (5H4) [TA336923] - Western blot analysis using Sonic Hedgehog mAb against HEK293 (1) and Sonic Hedgehog (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Sonic Hedgehog Antibody (5H4) [TA336923] - Western blot analysis using Sonic Hedgehog mAb against human Sonic Hedgehog recombinant protein. (Expected MW is 41 kDa)
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot: Sonic Hedgehog Antibody (5H4) [TA336923] - Western blot analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- ELISA: Sonic Hedgehog Antibody (5H4) [TA336923] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Sonic Hedgehog Antibody (5H4) [TA336923] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow Cytometry: Sonic Hedgehog Antibody (5H4) [TA336923] - Flow cytometric analysis of HeLa cells using Sonic Hedgehog mouse mAb (green) and negative control (red).
- Validation comment
- FC
