LS-C359325

antibody from LSBio

Targeting: SHH HHG1, HLP3, HPE3, MCOPCB5, SMMCI, TPT, TPTPS

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C359325

Provider

LSBio

Product name

SHH / Sonic Hedgehog Antibody (aa26-170, clone 5H4) LS-C359325

Antibody type

Monoclonal

Description

Ascites

Reactivity

Human, Mouse, Simian

Host

Mouse

Isotype

IgG

Antibody clone number

5H4

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: Sonic Hedgehog Antibody (5H4) - Western blot analysis using Sonic Hedgehog mouse mAb against LNCaP (1), HepG2 (2), PANC-1 (3),HeLa (4), SK-N-SH (5), F9 (6), NIH3T3 (7), and COS7 (8) cell lysate.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: Sonic Hedgehog Antibody (5H4) - Western blot analysis using Sonic Hedgehog mAb against human Sonic Hedgehog recombinant protein. (Expected MW is 41 kDa)

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western Blot: Sonic Hedgehog Antibody (5H4) - Western blot analysis using Sonic Hedgehog mAb against HEK293 (1) and Sonic Hedgehog (AA: 26-161)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ELISA: Sonic Hedgehog Antibody (5H4) - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Sonic Hedgehog Antibody (5H4) - Immunohistochemical analysis of paraffin-embedded liver cancer tissues using Sonic Hedgehog mouse mAb with DAB staining.