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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP54013PU-N - Provider product page
- Provider
- OriGene
- Product name
- SPECC1L (Center) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- SPECC1L (Center) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SPECC1L
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of SPECC1 Antibody (Center) ??in HL-60 cell line lysates (35ug/lane). This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of SPECC1 Antibody (Center) ??in Mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of SPECC1 Antibody (Center) ??with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin fixed, paraffin embedded human brain tissue stained with SPECC1 Antibody (Center) ??followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HL-60 cells using SPECC1 Antibody (Center) ??(right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
