25390-1-AP

antibody from Invitrogen Antibodies

Targeting: SPECC1L CYTSA, KIAA0376

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

25390-1-AP

Provider

Invitrogen Antibodies

Product name

CYTSA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MKKASRSVG SVPKVSAISK TQTAEKIKPE NSSSASTGGK LVKPGTAASL SKTKSSDDLL AGMAGGVTVT NGVKGKKSTC PSAAPSASAP AMTTVENKSK ISTGTASSTK RSTSTGNKES SSTRERLRER TRLNQSKKLP SAGQGANDMA LAKRSRSRTA TECDVRMSKS KSDNQISDRA ALEAKVKDLL TLAKTKDVEI LHLRNELRDM RAQLGINEDH SEGDEKSEKE TIMAHQPTDV ESTLLQLQEQ NTAIREELNQ LKNENRMLKD RLNALGFSLE QRLDNSEKLF GYQSLSPEIT PGNQSDGGGT LTSSVEGSAP GSVEDLLSQD ENTLMDHQHS NSMDNLDSEC SEV (1-352 aa encoded by BC136471)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.28 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25390-1-AP (CYTSA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25390-1-AP ( CYTSA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells using 25390-1-AP (CYTSA antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 25390-1-AP ( CYTSA Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 25390-1-AP ( CYTSA Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using (Product # 25390-1-AP) (CYTSA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CYTSA (IP:25390-1-AP, 4ug; Detection:25390-1-AP 1:1000) with mouse brain tissue lysate 4000ug.