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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA502529 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502529, RRID:AB_11127045
- Product name
- KCTD14 mouse monoclonal antibody, clone OTI3A4 (formerly 3A4)
- Antibody type
- Monoclonal
- Description
- KCTD14 mouse monoclonal antibody, clone OTI3A4 (formerly 3A4)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KCTD14
- Isotype
- IgG
- Antibody clone number
- OTI3A4
- Vial size
- 100 µl
- Concentration
- 0.8 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KCTD14 (RC200745, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KCTD14.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-KCTD14 mouse monoclonal antibody (TA502529) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KCTD14(RC200745).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-KCTD14 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502529)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC200745 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-KCTD14 antibody(TA502529), and then analyzed by flow cytometry.
- Validation comment
- FC
