AF1300

antibody from Novus Biologicals

Targeting: MICA PERB11.1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF1300

Provider

Novus Biologicals

Product name

Goat Polyclonal MICA Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human MICA in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human MICB is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Homo-oligomerization of the activating natural killer cell receptor NKp30 ectodomain increases its binding affinity for cellular ligands.

Herrmann J, Berberich H, Hartmann J, Beyer S, Davies K, Koch J
The Journal of biological chemistry 2014 Jan 10;289(2):765-77

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Compartmental localization and clinical relevance of MICA antibodies after renal transplantation.

Li L, Chen A, Chaudhuri A, Kambham N, Sigdel T, Chen R, Sarwal MM
Transplantation 2010 Feb 15;89(3):312-9

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Interferon-gamma down-regulates NKG2D ligand expression and impairs the NKG2D-mediated cytolysis of MHC class I-deficient melanoma by natural killer cells.

Schwinn N, Vokhminova D, Sucker A, Textor S, Striegel S, Moll I, Nausch N, Tuettenberg J, Steinle A, Cerwenka A, Schadendorf D, Paschen A
International journal of cancer 2009 Apr 1;124(7):1594-604

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NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.

Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Molecular immunology 2008 Sep;45(15):3917-25

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An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.

Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socié G, Toubert A
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):2227-37

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An unusual CD56(bright) CD16(low) NK cell subset dominates the early posttransplant period following HLA-matched hematopoietic stem cell transplantation.

Dulphy N, Haas P, Busson M, Belhadj S, Peffault de Latour R, Robin M, Carmagnat M, Loiseau P, Tamouza R, Scieux C, Rabian C, Di Santo JP, Charron D, Janin A, Socié G, Toubert A
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 1;181(3):2227-37

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Human tumor-derived exosomes selectively impair lymphocyte responses to interleukin-2.

Clayton A, Mitchell JP, Court J, Mason MD, Tabi Z
Cancer research 2007 Aug 1;67(15):7458-66

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

MICA in Human Breast Cancer Tissue. MICA was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Goat Anti-Human MICA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1300) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.