Antibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Flow cytometry [2]
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- Product number
- MAB1300-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MICA Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MICA in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human MICB is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 159227
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Race-associated expression of MHC class I polypeptide-related sequence A (MICA) in prostate cancer.
The homeobox transcription factor MEIS2 is a regulator of cancer cell survival and IMiDs activity in Multiple Myeloma: modulation by Bromodomain and Extra-Terminal (BET) protein inhibitors.
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
NK-cell receptors NKp46 and NCR1 control human metapneumovirus infection.
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study.
NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
RNA-binding proteins regulate the expression of the immune activating ligand MICB.
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
MicroRNA editing facilitates immune elimination of HCMV infected cells.
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells.
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Sakiyama MJ, Espinoza I, Reddy A, de Carlo F, Kumar A, Levenson AS, Bae S, Zhou X, Claudio PP, Lewin J, Manucha V, Pound CR, Vijayakumar S, Gomez CR
Experimental and molecular pathology 2019 Jun;108:173-182
Experimental and molecular pathology 2019 Jun;108:173-182
The homeobox transcription factor MEIS2 is a regulator of cancer cell survival and IMiDs activity in Multiple Myeloma: modulation by Bromodomain and Extra-Terminal (BET) protein inhibitors.
Abruzzese MP, Bilotta MT, Fionda C, Zingoni A, Soriani A, Petrucci MT, Ricciardi MR, Molfetta R, Paolini R, Santoni A, Cippitelli M
Cell death & disease 2019 Apr 11;10(4):324
Cell death & disease 2019 Apr 11;10(4):324
Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition.
Raneros AB, Minguela A, Rodriguez RM, Colado E, Bernal T, Anguita E, Mogorron AV, Gil AC, Vidal-Castiñeira JR, Márquez-Kisinousky L, Bulnes PD, Marin AM, Garay MCG, Suarez-Alvarez B, Lopez-Larrea C
Oncotarget 2017 May 9;8(19):31959-31976
Oncotarget 2017 May 9;8(19):31959-31976
MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells.
Breunig C, Pahl J, Küblbeck M, Miller M, Antonelli D, Erdem N, Wirth C, Will R, Bott A, Cerwenka A, Wiemann S
Cell death & disease 2017 Aug 3;8(8):e2973
Cell death & disease 2017 Aug 3;8(8):e2973
Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20.
Charpak-Amikam Y, Kubsch T, Seidel E, Oiknine-Djian E, Cavaletto N, Yamin R, Schmiedel D, Wolf D, Gribaudo G, Messerle M, Cicin-Sain L, Mandelboim O
Scientific reports 2017 Aug 17;7(1):8661
Scientific reports 2017 Aug 17;7(1):8661
NK-cell receptors NKp46 and NCR1 control human metapneumovirus infection.
Diab M, Glasner A, Isaacson B, Bar-On Y, Drori Y, Yamin R, Duev-Cohen A, Danziger O, Zamostiano R, Mandelboim M, Jonjic S, Bacharach E, Mandelboim O
European journal of immunology 2017 Apr;47(4):692-703
European journal of immunology 2017 Apr;47(4):692-703
Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells.
Son CH, Lee HR, Koh EK, Shin DY, Bae JH, Yang K, Park YS
Scientific reports 2016 Sep 27;6:32470
Scientific reports 2016 Sep 27;6:32470
Novel chemoimmunotherapeutic strategy for hepatocellular carcinoma based on a genome-wide association study.
Goto K, Annan DA, Morita T, Li W, Muroyama R, Matsubara Y, Ito S, Nakagawa R, Tanoue Y, Jinushi M, Kato N
Scientific reports 2016 Dec 2;6:38407
Scientific reports 2016 Dec 2;6:38407
NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
Nielsen MM, Dyring-Andersen B, Schmidt JD, Witherden D, Lovato P, Woetmann A, Ødum N, Poulsen SS, Havran WL, Geisler C, Bonefeld CM
The Journal of investigative dermatology 2015 May;135(5):1311-1319
The Journal of investigative dermatology 2015 May;135(5):1311-1319
Coordinated expression of DNAM-1 and LFA-1 in educated NK cells.
Enqvist M, Ask EH, Forslund E, Carlsten M, Abrahamsen G, Béziat V, Andersson S, Schaffer M, Spurkland A, Bryceson Y, Önfelt B, Malmberg KJ
Journal of immunology (Baltimore, Md. : 1950) 2015 May 1;194(9):4518-27
Journal of immunology (Baltimore, Md. : 1950) 2015 May 1;194(9):4518-27
Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci MT, Ricciardi MR, Fuerst D, Amendola MG, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
Journal of immunology (Baltimore, Md. : 1950) 2015 Jul 15;195(2):736-48
Journal of immunology (Baltimore, Md. : 1950) 2015 Jul 15;195(2):736-48
Metastatic colon cancer cell populations contain more cancer stem-like cells with a higher susceptibility to natural killer cell-mediated lysis compared with primary colon cancer cells.
Kim GR, Ha GH, Bae JH, Oh SO, Kim SH, Kang CD
Oncology letters 2015 Apr;9(4):1641-1646
Oncology letters 2015 Apr;9(4):1641-1646
Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
Fielding CA, Aicheler R, Stanton RJ, Wang EC, Han S, Seirafian S, Davies J, McSharry BP, Weekes MP, Antrobus PR, Prod'homme V, Blanchet FP, Sugrue D, Cuff S, Roberts D, Davison AJ, Lehner PJ, Wilkinson GW, Tomasec P
PLoS pathogens 2014 May;10(5):e1004058
PLoS pathogens 2014 May;10(5):e1004058
RNA-binding proteins regulate the expression of the immune activating ligand MICB.
Nachmani D, Gutschner T, Reches A, Diederichs S, Mandelboim O
Nature communications 2014 Jun 13;5:4186
Nature communications 2014 Jun 13;5:4186
A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells.
Robinet P, Baychelier F, Fontaine T, Picard C, Debré P, Vieillard V, Latgé JP, Elbim C
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5332-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5332-42
Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.
Soriani A, Iannitto ML, Ricci B, Fionda C, Malgarini G, Morrone S, Peruzzi G, Ricciardi MR, Petrucci MT, Cippitelli M, Santoni A
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):950-60
MicroRNA editing facilitates immune elimination of HCMV infected cells.
Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le VT, Galun E, Horejsi V, Isakov O, Shomron N, Wolf DG, Hengel H, Mandelboim O
PLoS pathogens 2014 Feb;10(2):e1003963
PLoS pathogens 2014 Feb;10(2):e1003963
Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
Nielsen N, Ødum N, Ursø B, Lanier LL, Spee P
PloS one 2012;7(2):e31959
PloS one 2012;7(2):e31959
MiR-10b downregulates the stress-induced cell surface molecule MICB, a critical ligand for cancer cell recognition by natural killer cells.
Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
Cancer research 2012 Nov 1;72(21):5463-72
Cancer research 2012 Nov 1;72(21):5463-72
NK cells from HCV-infected patients effectively induce apoptosis of activated primary human hepatic stellate cells in a TRAIL-, FasL- and NKG2D-dependent manner.
Glässner A, Eisenhardt M, Krämer B, Körner C, Coenen M, Sauerbruch T, Spengler U, Nattermann J
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Laboratory investigation; a journal of technical methods and pathology 2012 Jul;92(7):967-77
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
Oncogene 2011 Jun 9;30(23):2622-32
Oncogene 2011 Jun 9;30(23):2622-32
Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
Björkström NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaëlsson J, Malmberg KJ, Klingström J, Ahlm C, Ljunggren HG
The Journal of experimental medicine 2011 Jan 17;208(1):13-21
The Journal of experimental medicine 2011 Jan 17;208(1):13-21
Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells.
Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
PloS one 2011 Feb 25;6(2):e16899
PloS one 2011 Feb 25;6(2):e16899
NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
Molecular immunology 2008 Sep;45(15):3917-25
Molecular immunology 2008 Sep;45(15):3917-25
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MICA in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MICA Specificity is Shown by Flow Cytometry in Knockout Cell Line. MICA knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICA Monoclonal Antibody (Catalog # MAB1300, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the MICA knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.