Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB13001-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human MICA/B Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MICA/B in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q29983
- Isotype
- IgG
- Antibody clone number
- 159207
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Lower protein expression levels of MHC class I chain-related gene A in hepatocellular carcinoma are at high risk of recurrence after surgical resection.
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis.
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells.
Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
Huang CF, Wang SC, Chang WT, Yeh ML, Huang CI, Lin ZY, Chen SC, Chuang WL, Huang JF, Dai CY, Chen YL, Yu ML
Scientific reports 2018 Oct 25;8(1):15821
Scientific reports 2018 Oct 25;8(1):15821
Umbilical cord blood plasma contains soluble NKG2D ligands that mediate loss of natural killer cell function and cytotoxicity.
Cox ST, Laza-Briviesca R, Pearson H, Soria B, Gibson D, Gomez S, Madrigal JA, Saudemont A
European journal of immunology 2015 Aug;45(8):2324-34
European journal of immunology 2015 Aug;45(8):2324-34
Hepatitis C virus impairs natural killer cell-mediated augmentation of complement synthesis.
Kim H, Bose SK, Meyer K, Ray R
Journal of virology 2014 Mar;88(5):2564-71
Journal of virology 2014 Mar;88(5):2564-71
Human Herpesvirus 8 (HHV8) sequentially shapes the NK cell repertoire during the course of asymptomatic infection and Kaposi sarcoma.
Dupuy S, Lambert M, Zucman D, Choukem SP, Tognarelli S, Pages C, Lebbé C, Caillat-Zucman S
PLoS pathogens 2012 Jan;8(1):e1002486
PLoS pathogens 2012 Jan;8(1):e1002486
Mutations of the von Hippel-Lindau gene confer increased susceptibility to natural killer cells of clear-cell renal cell carcinoma.
Perier A, Fregni G, Wittnebel S, Gad S, Allard M, Gervois N, Escudier B, Azzarone B, Caignard A
Oncogene 2011 Jun 9;30(23):2622-32
Oncogene 2011 Jun 9;30(23):2622-32
Thermal- and oxidative stress causes enhanced release of NKG2D ligand-bearing immunosuppressive exosomes in leukemia/lymphoma T and B cells.
Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
PloS one 2011 Feb 25;6(2):e16899
PloS one 2011 Feb 25;6(2):e16899
Transfer of NKG2D and MICB at the cytotoxic NK cell immune synapse correlates with a reduction in NK cell cytotoxic function.
Roda-Navarro P, Vales-Gomez M, Chisholm SE, Reyburn HT
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 25;103(30):11258-63
Proceedings of the National Academy of Sciences of the United States of America 2006 Jul 25;103(30):11258-63
Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
Cancer research 2005 Dec 1;65(23):11136-45
Cancer research 2005 Dec 1;65(23):11136-45
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MICA/B in Human Pancreas. MICA/B was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of MICA/B in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICA/B Monoclonal Antibody (Catalog # MAB13001, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.