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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA325054 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal MICA Antibody (Center)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal MICA Antibody (Center)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- MICA
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.46 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of MICA Antibody (Center) (Cat. #TA325054) in MDA-MB231 cell line lysates (35ug/lane). MICA (arrow) was detected using the purified Pab.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- MICA Antibody (Center) (Cat.# TA325054) western blot analysis in MDA-MB-231 cell line lysates (35ug/lane).This demonstrates the MICA antibody detected the MICA protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of MICA Antibody (Center)(Cat#TA325054) with MDA-MB231 cell followed by Alexa Fluor?????488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma with MICA Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of SK-Br-3 cells using MICA Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
